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Expression of two insecticidal genes in Cotton - Allah Bakhsh#Tayyab Husnain
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2011, ISBN: 9783844389203

ID: 862597564

Spatio-temporal expression of insecticidal gene (Cry1Ac& Cry2A) in pre existing lines of transgenic cotton was studied. Seasonal decline in expression of genes differed significantly among different cotton lines tested in the field conditions. The leaves of the Bt cotton plants were found to have the highest levels of toxin expression followed by squares, bolls, anthers and petals. Expression of the genes decreased consistently with the age of plants. Toxin expression in fruiting parts was not enough to confer full resistance against bollworms. The reduction in efficacy of transgenic cotton plants late in the season was attributed to reduction in promoter activity. For this purpose, Rubisco small subunit promoter was isolated from Gossypium arboreum that was further cloned upstream of Cry1Ac in expression vector pCAMBIA 1301. A local cotton cultivar was transformed with Cry1Ac driven by rbcS promoter. The same cotton cultivar was also transformed with Cry1Ac gene driven by 35SCaMV promoter to compare the expression pattern of insecticidal gene under two different promoters. The results showed that rbcS is an efficient promoter to drive Bt gene expression in cotton. Development of Transgenic Cotton with sustained resistance against targeted insect pests Buch (fremdspr.) Taschenbuch 01.12.2011 Bücher>Fremdsprachige Bücher>Englische Bücher, LAP Lambert Academic Publishing, .201

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Expression of two insecticidal genes in Cotton - Allah Bakhsh#Tayyab Husnain
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Expression of two insecticidal genes in Cotton - neues Buch

ISBN: 9783844389203

ID: 836835298

Spatio-temporal expression of insecticidal gene (Cry1Ac& Cry2A) in pre existing lines of transgenic cotton was studied. Seasonal decline in expression of genes differed significantly among different cotton lines tested in the field conditions. The leaves of the Bt cotton plants were found to have the highest levels of toxin expression followed by squares, bolls, anthers and petals. Expression of the genes decreased consistently with the age of plants. Toxin expression in fruiting parts was not enough to confer full resistance against bollworms. The reduction in efficacy of transgenic cotton plants late in the season was attributed to reduction in promoter activity. For this purpose, Rubisco small subunit promoter was isolated from Gossypium arboreum that was further cloned upstream of Cry1Ac in expression vector pCAMBIA 1301. A local cotton cultivar was transformed with Cry1Ac driven by rbcS promoter. The same cotton cultivar was also transformed with Cry1Ac gene driven by 35SCaMV promoter to compare the expression pattern of insecticidal gene under two different promoters. The results showed that rbcS is an efficient promoter to drive Bt gene expression in cotton. Development of Transgenic Cotton with sustained resistance against targeted insect pests Buch (fremdspr.) Bücher>Fremdsprachige Bücher>Englische Bücher, LAP Lambert Academic Publishing

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Expression of two insecticidal genes in Cotton - Tayyab Husnain#Allah Bakhsh
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Expression of two insecticidal genes in Cotton - neues Buch

ISBN: 9783844389203

ID: 104858092

Spatio-temporal expression of insecticidal gene (Cry1Ac& Cry2A) in pre existing lines of transgenic cotton was studied. Seasonal decline in expression of genes differed significantly among different cotton lines tested in the field conditions. The leaves of the Bt cotton plants were found to have the highest levels of toxin expression followed by squares, bolls, anthers and petals. Expression of the genes decreased consistently with the age of plants. Toxin expression in fruiting parts was not enough to confer full resistance against bollworms. The reduction in efficacy of transgenic cotton plants late in the season was attributed to reduction in promoter activity. For this purpose, Rubisco small subunit promoter was isolated from Gossypium arboreum that was further cloned upstream of Cry1Ac in expression vector pCAMBIA 1301. A local cotton cultivar was transformed with Cry1Ac driven by rbcS promoter. The same cotton cultivar was also transformed with Cry1Ac gene driven by 35SCaMV promoter to compare the expression pattern of insecticidal gene under two different promoters. The results showed that rbcS is an efficient promoter to drive Bt gene expression in cotton. Development of Transgenic Cotton with sustained resistance against targeted insect pests Buch (fremdspr.) Bücher>Fremdsprachige Bücher>Englische Bücher, LAP Lambert Academic Publishing

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Expression of two insecticidal genes in Cotton als Buch von Allah Bakhsh, Tayyab Husnain - Allah Bakhsh, Tayyab Husnain
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ISBN: 9783844389203

ID: 696367148

Expression of two insecticidal genes in Cotton:Development of Transgenic Cotton with sustained resistance against targeted insect pests Allah Bakhsh, Tayyab Husnain Expression of two insecticidal genes in Cotton:Development of Transgenic Cotton with sustained resistance against targeted insect pests Allah Bakhsh, Tayyab Husnain Bücher > Wissenschaft > Umweltwissenschaft, LAP Lambert Academic Publishing

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Expression of two insecticidal genes in Cotton als Buch von Allah Bakhsh, Tayyab Husnain - LAP Lambert Academic Publishing
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Expression of two insecticidal genes in Cotton ab 68 EURO Development of Transgenic Cotton with sustained resistance against targeted insect pests Expression of two insecticidal genes in Cotton ab 68 EURO Development of Transgenic Cotton with sustained resistance against targeted insect pests Bücher > Wissenschaft > Umweltwissenschaft

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EAN (ISBN-13): 9783844389203
ISBN (ISBN-10): 3844389202
Gebundene Ausgabe
Taschenbuch
Erscheinungsjahr: 2011
Herausgeber: AV Akademikerverlag GmbH & Co. KG.

Buch in der Datenbank seit 12.11.2008 11:42:33
Buch zuletzt gefunden am 17.11.2017 22:58:10
ISBN/EAN: 9783844389203

ISBN - alternative Schreibweisen:
3-8443-8920-2, 978-3-8443-8920-3


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