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Directed Evolution Library Creation - Frances H. Arnold
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Directed Evolution Library Creation: Paperback: Humana Press Inc. : 9781617374715: 28 Oct 2010: Biological systems are very special substrates for engineering?uniquely the products of evolution, they are easily redesigned by similar approaches. A simple algorithm of iterative cycles of diversification and selection, evolution works at all scales, from single molecules to whole ecosystems. In the little more than a decade since the first reported applications of evolutionary design to enz. Biological systems are very special substrates for engineering?uniquely the products of evolution, they are easily redesigned by similar approaches. A simple algorithm of iterative cycles of diversification and selection, evolution works at all scales, from single molecules to whole ecosystems. In the little more than a decade since the first reported applications of evolutionary design to enzyme engineering, directed evolution has matured to the point where it now represents the centerpiece of industrial biocatalyst development and is being practiced by thousands of academic and industrial scientists in com- nies and universities around the world. The appeal of directed evolution is easy to understand: it is conceptually straightforward, it can be practiced without any special instrumentation and, most important, it frequently yields useful solutions, many of which are totally unanticipated. Directed evolution has r- dered protein engineering readily accessible to a broad audience of scientists and engineers who wish to tailor a myriad of protein properties, including th- mal and solvent stability, enzyme selectivity, specific activity, protease s- ceptibility, allosteric control of protein function, ligand binding, transcriptional activation, and solubility. Furthermore, the range of applications has expanded to the engineering of more complex functions such as those performed by m- tiple proteins acting in concert (in biosynthetic pathways) or as part of mac- molecular complexes and biological networks. Cellular Biology (cytology), , , , Directed Evolution Library Creation, Frances H. Arnold, 9781617374715, Humana Press Inc., , , , ,

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Directed Evolution Library Creation: Methods and Protocols (Methods in Molecular Biology) - Frances H. Arnold (Editor), George Georgiou (Editor)
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Details zum Buch
Directed Evolution Library Creation: Methods and Protocols
Autor:

Arnold, Frances H. and Georgiou, George

Titel:

Directed Evolution Library Creation: Methods and Protocols

ISBN-Nummer:

A comprehensive compendium of cutting-edge protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologus recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Copy for Both Volumes Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function.

Detailangaben zum Buch - Directed Evolution Library Creation: Methods and Protocols


EAN (ISBN-13): 9781617374715
ISBN (ISBN-10): 1617374717
Gebundene Ausgabe
Taschenbuch
Erscheinungsjahr: 2010
Herausgeber: SPRINGER VERLAG GMBH
232 Seiten
Gewicht: 0,336 kg
Sprache: eng/Englisch

Buch in der Datenbank seit 09.07.2011 21:08:40
Buch zuletzt gefunden am 08.04.2017 20:19:54
ISBN/EAN: 9781617374715

ISBN - alternative Schreibweisen:
1-61737-471-7, 978-1-61737-471-5


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