ISBN: 9781493926879
This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISP… Mehr…
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ISBN: 9781493926879
This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR… Mehr…
| Springer.com new in stock. Versandkosten:zzgl. Versandkosten. Details... |
ISBN: 9781493926879
Biomedicine; Gene Expression CRISPR-Cas defense systems, CRISPR-Cas9 technology, CRISPR-based genetic tools, DNA/protein, RNA/protein, archaeal species, bacterial species, genetic element… Mehr…
| Springer.com Versandkosten: EUR 0.00 Details... |
ISBN: 9781493926879
Biomedicine; Gene Expression CRISPR-Cas defense systems, CRISPR-Cas9 technology, CRISPR-based genetic tools, DNA/protein, RNA/protein, archaeal species, bacterial species, genetic element… Mehr…
| Springer.com Versandkosten:zzgl. Versandkosten. Details... |
2015, ISBN: 9781493926879
Methods and Protocols, eBooks, eBook Download (EPUB), [PU: Springer New York], Springer New York, 2015
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ISBN: 9781493926879
This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISP… Mehr…
ISBN: 9781493926879
This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR… Mehr…
ISBN: 9781493926879
Biomedicine; Gene Expression CRISPR-Cas defense systems, CRISPR-Cas9 technology, CRISPR-based genetic tools, DNA/protein, RNA/protein, archaeal species, bacterial species, genetic element… Mehr…
ISBN: 9781493926879
Biomedicine; Gene Expression CRISPR-Cas defense systems, CRISPR-Cas9 technology, CRISPR-based genetic tools, DNA/protein, RNA/protein, archaeal species, bacterial species, genetic element… Mehr…
2015, ISBN: 9781493926879
Methods and Protocols, eBooks, eBook Download (EPUB), [PU: Springer New York], Springer New York, 2015
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Detailangaben zum Buch - CRISPR
EAN (ISBN-13): 9781493926879
Erscheinungsjahr: 2015
Herausgeber: Springer New York
Buch in der Datenbank seit 2017-01-27T17:58:30+01:00 (Berlin)
Detailseite zuletzt geändert am 2023-07-17T23:30:59+02:00 (Berlin)
ISBN/EAN: 9781493926879
ISBN - alternative Schreibweisen:
978-1-4939-2687-9
Alternative Schreibweisen und verwandte Suchbegriffe:
Autor des Buches: charpentier, lundgren
Daten vom Verlag:
Autor/in: Magnus Lundgren; Emmanuelle Charpentier; Peter C. Fineran
Titel: Methods in Molecular Biology; CRISPR - Methods and Protocols
Verlag: Humana; Springer US
366 Seiten
Erscheinungsjahr: 2015-05-18
New York; NY; US
Sprache: Englisch
117,69 € (DE)
121,00 € (AT)
142,00 CHF (CH)
Available
XIV, 366 p. 63 illus., 37 illus. in color.
EA; E107; eBook; Nonbooks, PBS / Biologie/Genetik, Gentechnik; Genetik (nicht-medizinisch); Verstehen; CRISPR-Cas defense systems; CRISPR-Cas9 technology; CRISPR-based genetic tools; DNA/protein; RNA/protein; archaeal species; bacterial species; genetic elements; horizontal gene transfer; protein/protein interactions; gene expression; B; Molecular Genetics; Biomedical and Life Sciences; BB
Investigating CRISPR RNA Biogenesis and Function using RNA-seq.- In vitro Co-reconstitution of Cas Protein Complexes.- Analysis of CRISPR pre-crRNA Cleavage.- Annotation and Classification of CRISPR-Cas Systems.- Computational Detection of CRISPR/crRNA Targets.- High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium.- Spacer-based Macroarrays for CRISPR Genotyping.- Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS).- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR.- Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli.- Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes.- Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus.- Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids .- Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition.- Using the CRISPR-Cas system to Positively Select Mutants In Genes Essential for its Functionl.- Analysis of Nuclease Activity of Cas1 Proteins Against Complex DNA Substrates.- Characterizing Metal-Dependent Nucleases of CRISPR-Cas prokaryotic Adaptive Immunity Systems.- Cas3 Nuclease-Helicase Activity Assays.- Chemical and Enzymatic Footprint Analyses of R-loop Formation by Cascade-crRNA Complex.- Creation andAnalysis of a Virome: Using CRISPR Spacers.- Targeted Mutagenesis in Zebrafish using CRISPR RNA-guided Nucleases.- Precise Genome Editing of Drosophila with CRISPR RNA-guided Cas9.- Targeted Transcriptional Repression in Bacteria using CRISPR Interference (CRISPRi).Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
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