Laboratory Directions For Beginners In Bacteriology - Taschenbuch

LABORATORY DIRECTIONS BEGINNERS IN BACTERIOLOGY VERANUS A. MOORE, B.S., M.D. Professor of Comparative Pathology and Bacteriology and of Meat Inspection, N. Y. State Veterinary College, Cornell University, Ithaca, N. Y. -- 1898. -- PREFATORY NOTE. It has been found desirable to provide the student, just beginning the study of bacteriology, with a somewhat detailed outline of the work to be done at each laboratory sekion. The selecting of the particular things to be done and the choosing of methods to be followed are difficult tasks. The assigning of diretions for doing work under assumed conditions must necessarily partake of the empirical, and often fail. It is evident, himever, hat practical bhcteriology must, if successfully taught, be cast in a somewhat definite form in order that the student may come to a knowledge of the fundamental principles underlying the subject in its twofold capacity, that of a pure science and of a useful art. These outlines are intended to serve simply as a guide through an introductory laboratory course preparatory either to independent research work, or to form the basis for the application of the principles of bacteriology in the practice of human or of comparative medicine. They aim to impart a technical and working knowledge of certain of the more essential methods and to develop a definite knowledge of a few important species of bacteria. During the past year, they were furnished the students in mimeographed sheets, but after making the changes suggested by this application it seems desirable to put them in a more convenient form. In adjusting the amount of work for each exercise to the necessary limitations of time and facilities, I am indebted toMr. Raymond C. Reed, Instructor in this Department, for much valuable assistance. I wish also to thank Prof. Charles Wright Dodge of the University of Rochester for helpful suggestions. Should these outlines fall in the hands of other teachers or workers in this subject, criticisms are cordially invited. V. A. M. . CONTENTS. PARTIAL LIST OF TEXT AND REFERENCE BOOKS.-APPA- RATUS AND MATERIAL.-LABORATORY MAXIMS. 111. IV. v. VI. VII. VIII. IX. X. XI. XII. XIII. XIV. xv. XVI. XVII. XVIII. XIX. XX. XXI. XXII. XXIII. XXIV. xxv. XXVI. XXVII. XXVIII. PAGE. Cleaning glassware - -, ---- -- ---- - ----- ------ ------ I I Plugging test tubes and flasks and sterilizing the glassware- - - - - - - - - - -- --- - - - - - -- ---- - - -- -- - - ---- I3 The preparation.of bouillon I4 The preparation of agar and gelatin ---------------- i 7 Inoculating tubes of bouillon, agar and gelatin----- 20 The examination of cultures ---------------------- 22 Making and staining cover-glass preparations and formulz for staining solutions ------------------ 25 Making plate and Esmarch roll cultures ------------ 29 The examination of plate cultures and making subcultures from colonies -------------------------- 32 The preparation of certain special media ..---------- 34 Inoculating special media and examining cultures-, 37 The examination of cultures on special media-----, 38 The examination of cultures continued 40 The classification of bacteria ---, ----- ---- ---- 41 Identifying genera among bacteria ----------------- 43 Studying the morphology of bacteria -------------- 44 . ...