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Into the Membrane and Beyond: Improving Membrane Protein Over-Expression in Escherichia coli - Mirjam Klepsch
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Mirjam Klepsch:
Into the Membrane and Beyond: Improving Membrane Protein Over-Expression in Escherichia coli - Taschenbuch

ISBN: 3848419696

Paperback, [EAN: 9783848419692], LAP LAMBERT Academic Publishing, LAP LAMBERT Academic Publishing, Book, [PU: LAP LAMBERT Academic Publishing], LAP LAMBERT Academic Publishing, Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. Unfortunately, over-expression of membrane proteins in E. coli is usually toxic. I have studied the effects of the expression of membrane proteins, including the human KDEL receptor, on BL21(DE3) and its derivatives. Saturation of the Sec translocon, a cytoplasmic membrane associated protein conducting channel that mediates the insertion/ biogenesis of membrane proteins, appeared to be the prime reason for the toxicity of membrane protein over-expression. The obtained data have resulted in many leads and ideas to further improve membrane protein over-expression yields in E. coli, e.g. we have engineered a plasmid (pLemo) that helps avoiding saturation of the Sec translocon upon membrane protein over-expression in E. coli., 21, Education & Reference, 11444, Almanacs & Yearbooks, 11448, Atlases & Maps, 2572, Careers, 11626, Catalogs & Directories, 69820, College & University, 11472, Consumer Guides, 11475, Dictionaries & Thesauruses, 11713, Encyclopedias, 11823, English as a Second Language, 11761, Etiquette, 11773, Foreign Language Study & Reference, 11880, Genealogy, 5267709011, Graduate School, 11902, Quotations, 5267708011, Schools & Teaching, 5267723011, Studying & Workbooks, 5267710011, Test Preparation, 11871, Trivia & Fun Facts, 11970, Words, Language & Grammar, 5267707011, Writing, Research & Publishing Guides, 1000, Subjects, 283155, Books, 13511, Biochemistry, 13469, Biological Sciences, 75, Science & Math, 1000, Subjects, 283155, Books

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Into the Membrane and Beyond: Improving Membrane Protein Over-Expression in Escherichia coli - Mirjam Klepsch
Vergriffenes Buch, derzeit bei uns nicht verfügbar.
(*)
Mirjam Klepsch:
Into the Membrane and Beyond: Improving Membrane Protein Over-Expression in Escherichia coli - Taschenbuch

ISBN: 3848419696

Paperback, [EAN: 9783848419692], LAP LAMBERT Academic Publishing, LAP LAMBERT Academic Publishing, Book, [PU: LAP LAMBERT Academic Publishing], LAP LAMBERT Academic Publishing, 125238031, General AAS, 277913, Biochemistry, 277912, Biological Sciences, 57, Science & Nature, 1025612, Subjects, 266239, Books, 125231031, General AAS, 277912, Biological Sciences, 57, Science & Nature, 1025612, Subjects, 266239, Books, 923006, Higher Education, 922526, Education, 278320, Mathematics, 57, Science & Nature, 1025612, Subjects, 266239, Books, 125228031, General AAS, 57, Science & Nature, 1025612, Subjects, 266239, Books, 125364031, General AAS, 570694, Biochemistry, 564340, Biology, 564334, Scientific, Technical & Medical, 1025612, Subjects, 266239, Books, 125362031, General AAS, 564340, Biology, 564334, Scientific, Technical & Medical, 1025612, Subjects, 266239, Books, 400530011, English, 400529011, Language (feature_browse-bin), 365481011, Refinements, 266239, Books, 492564011, Paperback, 492562011, Format (binding_browse-bin), 365481011, Refinements, 266239, Books, 182018031, Regular Size, 182016031, Font Size (format_browse-bin), 365481011, Refinements, 266239, Books

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Into the Membrane and Beyond - Klepsch, Mirjam
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Klepsch, Mirjam:
Into the Membrane and Beyond - Taschenbuch

ISBN: 3848419696

Gebundene Ausgabe, ID: 11803319

Improving Membrane Protein Over-Expression in Escherichia coli - Buch, gebundene Ausgabe, 88 S., Beilagen: Paperback, Erschienen: 2012 LAP Lambert Academic Publishing

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Into the Membrane and Beyond - Klepsch, Mirjam
Vergriffenes Buch, derzeit bei uns nicht verfügbar.
(*)
Klepsch, Mirjam:
Into the Membrane and Beyond - Taschenbuch

2012, ISBN: 3848419696

Gebundene Ausgabe, ID: 11803319

Improving Membrane Protein Over-Expression in Escherichia coli - Buch, gebundene Ausgabe, 88 S., Beilagen: Paperback, Erschienen: 2012 LAP Lambert Academic Publishing

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