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Electrophoresis in Practice - Reiner Westermeier
Vergriffenes Buch, derzeit bei uns nicht verfügbar.
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Reiner Westermeier:

Electrophoresis in Practice - neues Buch

ISBN: 9783527604449

ID: 9783527604449

A Guide to Methods and Applications of DNA and Protein Separations InhaltsangabeForeword.Preface.Abbreviations.Part I Fundamentals.Introduction.1 Electrophoresis.1.0 General.1.1 Electrophoresis in non-restrictive gels.1.2 Electrophoresis in restrictive gels.2 Isotachophoresis.2.1 Migration with the same speed.2.2 & #8220 Ion train& #8221 separation.2.3 Zone sharpening effect.2.4 Concentration regulation effect.3 Isoelectric focusing.3.1 Principles.3.2 Gels for IEF.3.3 Temperature.3.4 Controlling the pH gradient.3.5 The kinds of pH gradients.3.6 Protein detection in IEFgels.3.7 Preparative isoelectric focusing.3.8 Titration curve analysis.4 Blotting.4.1 Principle.4.2 Transfer methods.4.3 Blotting membranes.4.4 Buffers for electrophoretic transfers.4.5 General staining.4.6 Blocking.4.7 Specific detection.4.8 Protein sequencing.4.9 Transfer problems.5 Interpretation of electropherograms.5.1 Introduction.5.2 Image analysis.6 Proteome Analysis.6.1 General.6.2 Sample preparation.6.3 Two-dimensional electrophoresis.6.4 Detection techniques.6.5 Image analysis.6.6 Protein spot identification.6.7 Bioinformatics.6.8 Functional proteomics.7 Instrumentation.7.1 Current and voltage conditions.7.2 Power supply.7.3 Separation chambers.7.4 Staining apparatus for gels and blots.7.5 Automated electrophoresis.7.6 Instrumentsfor 2-D electrophoresis.7.7 Safety measures.7.8 Environmental aspects.Part II Equipment and Methods.Equipment for Part II.Instrumentation.Special laboratory equipment.Consumables.Chemicals.Method 1: PAGE of dyes.1 Sample preparation.2 Stock solutions.3 Preparing the casting cassette.4 Casting ultrathin-layer gels.5 Electrophoretic separation.Method 2: Agarose and immuno electrophoresis.1 Sample preparation.2 Stock solutions.3 Preparing the gels.4 Electrophoresis.5 Protein detection.Method 3: Titration curve analysis.1 Sample preparation.2 Stock solutions.3 Preparing the blank gels.4 Titration curve analysis.5 Coomassie and silver staining.6 Interpreting the curves.Method 4: Native PAGE in amphoteric buffers.1 Sample preparation.2 Stock solutions.3 Preparing the empty gels.4 Electrophoresis.5 Coomassie and silver staining.Method 5: Agarose IEF.1 Sample preparation.2 Preparing the agarose gel.3 Isoelectric focusing.4 Protein detection.Method 6: PAGIEF in rehydrated gels.1 Sample preparation.2 Stock solutions.3 Preparing the blank gels.4 Isoelectric focusing.5 Coomassie and silver staining.6 Perspectives.Method 7: Horizontal SDS-PAGE.1 Sample preparation.2 Stock solutions for gel preparation.3 Preparing the casting cassette.4 Gradient gel.5 Electrophoresis.6 Protein detection.7 Blotting.8 Perspectives.Method 8: Vertical PAGE.1 Sample preparation.2 Stock solutions.3 Single gel casting.4 Multiple gel casting.5 Electrophoresis.6 SDS electrophoresis electrophoresis.5 Strategy for SSCP analysis.Method 14: Denaturing gradient gel electrophoresis.1 Sample preparation.2 Rehydration solutions.3 Preparing the rehydration cassette.4 Rehydration.5 Electrophoresis.6 Silver staining.Method 15: Denaturing PAGE of DNA.1 Sample preparation.2 Solutions.3 Rehydration.4 Electrophoresis.5 Silver staining.Appendix Trouble-shooting.A1 Isoelectric focusing.A1.1 PAGIEFwith carrier ampholytes.A1.2 Agarose IEFwith carrier ampholytes.A1.3 Immobilized pH gradients.A2 SDS electrophoresis.A3 Vertical PAGE.A4 Semidry blotting.A5 2-D electrophoresis.A6 DNA electrophoresis.References. Electrophoresis in Practice: InhaltsangabeForeword.Preface.Abbreviations.Part I Fundamentals.Introduction.1 Electrophoresis.1.0 General.1.1 Electrophoresis in non-restrictive gels.1.2 Electrophoresis in restrictive gels.2 Isotachophoresis.2.1 Migration with the same speed.2.2 & #8220 Ion train& #8221 separation.2.3 Zone sharpening effect.2.4 Concentration regulation effect.3 Isoelectric focusing.3.1 Principles.3.2 Gels for IEF.3.3 Temperature.3.4 Controlling the pH gradient.3.5 The kinds of pH gradients.3.6 Protein detection in IEFgels.3.7 Preparative isoelectric focusing.3.8 Titration curve analysis.4 Blotting.4.1 Principle.4.2 Transfer methods.4.3 Blotting membranes.4.4 Buffers for electrophoretic transfers.4.5 General staining.4.6 Blocking.4.7 Specific detection.4.8 Protein sequencing.4.9 Transfer problems.5 Interpretation of electropherograms.5.1 Introduction.5.2 Image analysis.6 Proteome Analysis.6.1 General.6.2 Sample preparation.6.3 Two-dimensional electrophoresis.6.4 Detection techniques.6.5 Image analysis.6.6 Protein spot identification.6.7 Bioinformatics.6.8 Functional proteomics.7 Instrumentation.7.1 Current and voltage conditions.7.2 Power supply.7.3 Separation chambers.7.4 Staining apparatus for gels and blots.7.5 Automated electrophoresis.7.6 Instrumentsfor 2-D electrophoresis.7.7 Safety measures.7.8 Environmental aspects.Part II Equipment and Methods.Equipment for Part II.Instrumentation.Special laboratory equipment.Consumables.Chemicals.Method 1: PAGE of dyes.1 Sample preparation.2 Stock solutions.3 Preparing the casting cassette.4 Casting ultrathin-layer gels.5 Electrophoretic separation.Method 2: Agarose and immuno electrophoresis.1 Sample preparation.2 Stock solutions.3 Preparing the gels.4 Electrophoresis.5 Protein detection.Method 3: Titration curve analysis.1 Sample preparation.2 Stock solutions.3 Preparing the blank gels.4 Titration curve analysis.5 Coomassie and silver staining.6 Interpreting the curves.Method 4: Native PAGE in amphoteric buffers.1 Sample preparation.2 Stock solutions.3 Preparing the empty gels.4 Electrophoresis.5 Coomassie and silver staining.Method 5: Agarose IEF.1 Sample preparation.2 Preparing the agarose gel.3 Isoelectric focusing.4 Protein detection.Method 6: PAGIEF in rehydrated gels.1 Sample preparation.2 Stock solutions.3 Preparing the blank gels.4 Isoelectric focusing.5 Coomassie and silver staining.6 Perspectives.Method 7: Horizontal SDS-PAGE.1 Sample preparation.2 Stock solutions for gel preparation.3 Preparing the casting cassette.4 Gradient gel.5 Electrophoresis.6 Protein detection.7 Blotting.8 Perspectives.Method 8: Vertical PAGE.1 Sample preparation.2 Stock solutions.3 Single gel casting.4 Multiple gel casting.5 Electrophoresis.6 SDS electrophoresis electrophoresis.5 Strategy for SSCP analysis.Method 14: Denaturing gradient gel electrophoresis.1 Sample preparation.2 Rehydration solutions.3 Preparing the rehydration cassette.4 Rehydration.5 Electrophoresis.6 Silver staining.Method 15: Denaturing PAGE of DNA.1 Sample preparation.2 Solutions.3 Rehydration.4 Electrophoresis.5 Silver staining.Appendix Trouble-shooting.A1 Isoelectric focusing.A1.1 PAGIEFwith carrier ampholytes.A1.2 Agarose IEFwith carrier ampholytes.A1.3 Immobilized pH gradients.A2 SDS electrophoresis.A3 Vertical PAGE.A4 Semidry blotting.A5 2-D electrophoresis.A6 DNA electrophoresis.References. Analytical Chemistry Analytische Chemie Biologie / Allgemeines, Lexika Biowissenschaften Cell & Molecular Biology Chemie Chemische Analyse Chemistry Chromatographie / Trennverfahren Chromatography / Separation Techniques Elektrophorese Genomfor, Wiley-VCH

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Electrophoresis in Practice - Reiner Westermeier
Vergriffenes Buch, derzeit bei uns nicht verfügbar.
(*)

Reiner Westermeier:

Electrophoresis in Practice - neues Buch

ISBN: 9783527604449

ID: 9783527604449

A Guide to Methods and Applications of DNA and Protein Separations InhaltsangabeForeword.Preface.Abbreviations.Part I Fundamentals.Introduction.1 Electrophoresis.1.0 General.1.1 Electrophoresis in non-restrictive gels.1.2 Electrophoresis in restrictive gels.2 Isotachophoresis.2.1 Migration with the same speed.2.2 “ Ion train” separation.2.3 Zone sharpening effect.2.4 Concentration regulation effect.3 Isoelectric focusing.3.1 Principles.3.2 Gels for IEF.3.3 Temperature.3.4 Controlling the pH gradient.3.5 The kinds of pH gradients.3.6 Protein detection in IEFgels.3.7 Preparative isoelectric focusing.3.8 Titration curve analysis.4 Blotting.4.1 Principle.4.2 Transfer methods.4.3 Blotting membranes.4.4 Buffers for electrophoretic transfers.4.5 General staining.4.6 Blocking.4.7 Specific detection.4.8 Protein sequencing.4.9 Transfer problems.5 Interpretation of electropherograms.5.1 Introduction.5.2 Image analysis.6 Proteome Analysis.6.1 General.6.2 Sample preparation.6.3 Two-dimensional electrophoresis.6.4 Detection techniques.6.5 Image analysis.6.6 Protein spot identification.6.7 Bioinformatics.6.8 Functional proteomics.7 Instrumentation.7.1 Current and voltage conditions.7.2 Power supply.7.3 Separation chambers.7.4 Staining apparatus for gels and blots.7.5 Automated electrophoresis.7.6 Instrumentsfor 2-D electrophoresis.7.7 Safety measures.7.8 Environmental aspects.Part II Equipment and Methods.Equipment for Part II.Instrumentation.Special laboratory equipment.Consumables.Chemicals.Method 1: PAGE of dyes.1 Sample preparation.2 Stock solutions.3 Preparing the casting cassette.4 Casting ultrathin-layer gels.5 Electrophoretic separation.Method 2: Agarose and immuno electrophoresis.1 Sample preparation.2 Stock solutions.3 Preparing the gels.4 Electrophoresis.5 Protein detection.Method 3: Titration curve analysis.1 Sample preparation.2 Stock solutions.3 Preparing the blank gels.4 Titration curve analysis.5 Coomassie and silver staining.6 Interpreting the curves.Method 4: Native PAGE in amphoteric buffers.1 Sample preparation.2 Stock solutions.3 Preparing the empty gels.4 Electrophoresis.5 Coomassie and silver staining.Method 5: Agarose IEF.1 Sample preparation.2 Preparing the agarose gel.3 Isoelectric focusing.4 Protein detection.Method 6: PAGIEF in rehydrated gels.1 Sample preparation.2 Stock solutions.3 Preparing the blank gels.4 Isoelectric focusing.5 Coomassie and silver staining.6 Perspectives.Method 7: Horizontal SDS-PAGE.1 Sample preparation.2 Stock solutions for gel preparation.3 Preparing the casting cassette.4 Gradient gel.5 Electrophoresis.6 Protein detection.7 Blotting.8 Perspectives.Method 8: Vertical PAGE.1 Sample preparation.2 Stock solutions.3 Single gel casting.4 Multiple gel casting.5 Electrophoresis.6 SDS electrophoresis electrophoresis.5 Strategy for SSCP analysis.Method 14: Denaturing gradient gel electrophoresis.1 Sample preparation.2 Rehydration solutions.3 Preparing the rehydration cassette.4 Rehydration.5 Electrophoresis.6 Silver staining.Method 15: Denaturing PAGE of DNA.1 Sample preparation.2 Solutions.3 Rehydration.4 Electrophoresis.5 Silver staining.Appendix Trouble-shooting.A1 Isoelectric focusing.A1.1 PAGIEFwith carrier ampholytes.A1.2 Agarose IEFwith carrier ampholytes.A1.3 Immobilized pH gradients.A2 SDS electrophoresis.A3 Vertical PAGE.A4 Semidry blotting.A5 2-D electrophoresis.A6 DNA electrophoresis.References. Electrophoresis in Practice: InhaltsangabeForeword.Preface.Abbreviations.Part I Fundamentals.Introduction.1 Electrophoresis.1.0 General.1.1 Electrophoresis in non-restrictive gels.1.2 Electrophoresis in restrictive gels.2 Isotachophoresis.2.1 Migration with the same speed.2.2 “ Ion train” separation.2.3 Zone sharpening effect.2.4 Concentration regulation effect.3 Isoelectric focusing.3.1 Principles.3.2 Gels for IEF.3.3 Temperature.3.4 Controlling the pH gradient.3.5 The kinds of pH gradients.3.6 Protein detection in IEFgels.3.7 Preparative isoelectric focusing.3.8 Titration curve analysis.4 Blotting.4.1 Principle.4.2 Transfer methods.4.3 Blotting membranes.4.4 Buffers for electrophoretic transfers.4.5 General staining.4.6 Blocking.4.7 Specific detection.4.8 Protein sequencing.4.9 Transfer problems.5 Interpretation of electropherograms.5.1 Introduction.5.2 Image analysis.6 Proteome Analysis.6.1 General.6.2 Sample preparation.6.3 Two-dimensional electrophoresis.6.4 Detection techniques.6.5 Image analysis.6.6 Protein spot identification.6.7 Bioinformatics.6.8 Functional proteomics.7 Instrumentation.7.1 Current and voltage conditions.7.2 Power supply.7.3 Separation chambers.7.4 Staining apparatus for gels and blots.7.5 Automated electrophoresis.7.6 Instrumentsfor 2-D electrophoresis.7.7 Safety measures.7.8 Environmental aspects.Part II Equipment and Methods.Equipment for Part II.Instrumentation.Special laboratory equipment.Consumables.Chemicals.Method 1: PAGE of dyes.1 Sample preparation.2 Stock solutions.3 Preparing the casting cassette.4 Casting ultrathin-layer gels.5 Electrophoretic separation.Method 2: Agarose and immuno electrophoresis.1 Sample preparation.2 Stock solutions.3 Preparing the gels.4 Electrophoresis.5 Protein detection.Method 3: Titration curve analysis.1 Sample preparation.2 Stock solutions.3 Preparing the blank gels.4 Titration curve analysis.5 Coomassie and silver staining.6 Interpreting the curves.Method 4: Native PAGE in amphoteric buffers.1 Sample preparation.2 Stock solutions.3 Preparing the empty gels.4 Electrophoresis.5 Coomassie and silver staining.Method 5: Agarose IEF.1 Sample preparation.2 Preparing the agarose gel.3 Isoelectric focusing.4 Protein detection.Method 6: PAGIEF in rehydrated gels.1 Sample preparation.2 Stock solutions.3 Preparing the blank gels.4 Isoelectric focusing.5 Coomassie and silver staining.6 Perspectives.Method 7: Horizontal SDS-PAGE.1 Sample preparation.2 Stock solutions for gel preparation.3 Preparing the casting cassette.4 Gradient gel.5 Electrophoresis.6 Protein detection.7 Blotting.8 Perspectives.Method 8: Vertical PAGE.1 Sample preparation.2 Stock solutions.3 Single gel casting.4 Multiple gel casting.5 Electrophoresis.6 SDS electrophoresis electrophoresis.5 Strategy for SSCP analysis.Method 14: Denaturing gradient gel electrophoresis.1 Sample preparation.2 Rehydration solutions.3 Preparing the rehydration cassette.4 Rehydration.5 Electrophoresis.6 Silver staining.Method 15: Denaturing PAGE of DNA.1 Sample preparation.2 Solutions.3 Rehydration.4 Electrophoresis.5 Silver staining.Appendix Trouble-shooting.A1 Isoelectric focusing.A1.1 PAGIEFwith carrier ampholytes.A1.2 Agarose IEFwith carrier ampholytes.A1.3 Immobilized pH gradients.A2 SDS electrophoresis.A3 Vertical PAGE.A4 Semidry blotting.A5 2-D electrophoresis.A6 DNA electrophoresis.References. Analytical Chemistry Analytische Chemie Biologie / Allgemeines, Lexika Biowissenschaften Cell & Molecular Biology Chemie Chemische Analyse Chemistry Chromatographie / Trennverfahren Chromatography / Separation Techniques Elektrophorese Genomfor, Wiley-VCH

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Electrophoresis In Practice - Wiley
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Wiley:
Electrophoresis In Practice - neues Buch

2006

ISBN: 9783527604449

ID: 5601665

This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in USE. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference. This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in USE. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references. eBooks, , Electrophoresis In Practice~~EBook~~9783527604449~~Reiner Westermeier, , Electrophoresis In Practice, Reiner Westermeier, 9783527604449, Wiley, 03/06/2006, , , , Wiley

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Electrophoresis in Practice - A Guide to Methods and Applications of DNA and Protein Separations - Westermeier, Reiner
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Westermeier, Reiner:
Electrophoresis in Practice - A Guide to Methods and Applications of DNA and Protein Separations - neues Buch

2006, ISBN: 3527604448

ID: 9783527604449

In englischer Sprache. Verlag: Wiley-VCH, PART I: FUNDAMENTALS Introduction Electrophoresis Isotachophoresis Isoelectric Focusing Blotting Interpretation of Electropherograms Proteome Analysis Instrumentation PART II: EQUIPMENT AND METHODS Equipment for Part II Method 1: PAGE of Dyes Method 2: Agarose and Immuno Electrophoresis Method 3: Titration Curve Analysis Method 4: Native PAGE in Amphoteric Buffers Method 5: Agarose IEF Method 6: PAGIEF in Rehydrated Gels Method 7: Horizontal SDS-PAGE Method 8: Vertical PAGE Method 9: Semi-dry Blotting of Proteins Method 10: IEF in Immobilized pH Gradients Method 11: High-resolution 2D Electrophoresis Method 12: PAGE of Double Stranded DNA Method 13: Native PAGE of Single Stranded DNA Method 14: Denaturing Gradient Gel Electrophoresis Method 15: Denaturing PAGE of DNA Appendix A: Trouble-shooting References Index, PC-PDF, 426 Seiten, XX Seiten, 406 Seiten, 4., überarb. u. erw. Auflage, [GR: 9650 - Nonbooks, PBS / Chemie], [SW: - Chemie ], [Ausgabe: 4][PU:Wiley-VCH], [PU: Wiley-VCH, Weinheim]

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Electrophoresis in Practice - Reiner Westermeier
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Reiner Westermeier:
Electrophoresis in Practice - Erstausgabe

2006, ISBN: 9783527604449

ID: 24730103

[ED: 1], Auflage, eBook Download (PDF), eBooks, [PU: Wiley-Blackwell]

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