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Kleinster Preis: 93.46 EUR, größter Preis: 156.20 EUR, Mittelwert: 121.85 EUR
Directed Evolution Library Creation: Methods and Protocols - Frances H. Arnold (Editor), George Georgiou (Editor)
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ISBN: 9781617374715

ID: 9781617374715

Directed protein evolution-a powerful technique for the discovery of new enzymes and therapeutic proteins-has matured to the point that it is now the centerpiece of enzyme engineering, where it has catalyzed the development of numerous experimental methods. In Directed Evolution Library Creation: Methods and Prools, seasoned practitioners from many leading laboratories share their best experimental prools for the generation of molecular diversity. Described in step-by-step detail to Directed protein evolution-a powerful technique for the discovery of new enzymes and therapeutic proteins-has matured to the point that it is now the centerpiece of enzyme engineering, where it has catalyzed the development of numerous experimental methods. In Directed Evolution Library Creation: Methods and Prools, seasoned practitioners from many leading laboratories share their best experimental prools for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these prools include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologous recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various prools for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Taken together, Directed Evolution Library Creation: Methods and Prools and Directed Enzyme Evolution: Screening and Selection Methods capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications. Textbooks New, Books~~Medicine~~Genetics, Directed-Evolution-Library-Creation~~Frances-H-Arnold, 999999999, Directed Evolution Library Creation: Methods and Protocols, Frances H. Arnold (Editor), George Georgiou (Editor), 1617374717, Springer-Verlag New York, LLC, , , , , Springer-Verlag New York, LLC

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Directed Evolution Library Creation (1st ed. Softcover of orig. ed. 2003) - Frances H. Arnold, George Georgiou
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Directed Evolution Library Creation (1st ed. Softcover of orig. ed. 2003) - Taschenbuch

2003, ISBN: 1617374717

ID: 5962577091

[EAN: 9781617374715], Neubuch, [PU: Humana Press Inc.], BRAND NEW PRINT ON DEMAND., Directed Evolution Library Creation (1st ed. Softcover of orig. ed. 2003), Frances H. Arnold, George Georgiou, A comprehensive compendium of cutting-edge protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologus recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Copy for Both Volumes Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function.

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Directed Evolution Library Creation: Methods and Protocols (Methods in Molecular Biology) - Frances H. Arnold, George Georgiou
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Directed Evolution Library Creation: Methods and Protocols (Methods in Molecular Biology) - Taschenbuch

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Taschenbuch, [EAN: 9781617374715], Humana Press, Humana Press, Book, [PU: Humana Press], Humana Press, 56087011, Biochemie, 56458011, Biowissenschaften, 56047011, Fachbücher, 54071011, Genres, 52044011, Fremdsprachige Bücher, 56182011, Molekularbiologie, 56458011, Biowissenschaften, 56047011, Fachbücher, 54071011, Genres, 52044011, Fremdsprachige Bücher, 56490011, Zellbiologie, 56458011, Biowissenschaften, 56047011, Fachbücher, 54071011, Genres, 52044011, Fremdsprachige Bücher, 56562011, Genetik, 56558011, Grundlagen, 56535011, Medizin, 54071011, Genres, 52044011, Fremdsprachige Bücher, 208623031, Taschenbuch, 208621031, Format (binding_browse-bin), 366250011, Refinements, 52044011, Fremdsprachige Bücher, 1621283031, POD, 425410031, Special Features Stores, 425408031, Self Service, 52044011, Fremdsprachige Bücher

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Directed Evolution Library Creation: Methods and Protocols - Arnold, Frances H. and Georgiou, George
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2014, ISBN: 1617374717

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Details zum Buch
Directed Evolution Library Creation: Methods and Protocols
Autor:

Arnold, Frances H. and Georgiou, George

Titel:

Directed Evolution Library Creation: Methods and Protocols

ISBN-Nummer:

9781617374715

A comprehensive compendium of cutting-edge protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologus recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Copy for Both Volumes Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function.

Detailangaben zum Buch - Directed Evolution Library Creation: Methods and Protocols


EAN (ISBN-13): 9781617374715
ISBN (ISBN-10): 1617374717
Taschenbuch
Erscheinungsjahr: 2010
Herausgeber: SPRINGER VERLAG GMBH
232 Seiten
Gewicht: 0,336 kg
Sprache: eng/Englisch

Buch in der Datenbank seit 09.07.2011 21:08:40
Buch zuletzt gefunden am 26.02.2015 00:55:18
ISBN/EAN: 9781617374715

ISBN - alternative Schreibweisen:
1-61737-471-7, 978-1-61737-471-5

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