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The Role of Lipid Rafts in Canine Distemper Virus Infection - Heidi Imhoff
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Heidi Imhoff:

The Role of Lipid Rafts in Canine Distemper Virus Infection - Taschenbuch

2008, ISBN: 3639046307

ID: 9852877483

[EAN: 9783639046304], Neubuch, [PU: Vdm Verlag Aug 2008], This item is printed on demand - Print on Demand Titel. - Cholesterol is known to play an important role in stabilisingparticular cellular membrane structures, so-called lipid rafts. Forseveral viruses a dependence on cholesterol for virus entry and/ormorphogenesis has been shown. Using flow cytometry and fluorescencemicroscopy we demonstrate that Canine distemper virus (CDV)infection of cells was not impaired after cellular cholesterol hadbeen depleted by the drug methyl-beta-cyclodextrin (MbCD). Thiseffect was independent of the multiplicity of infection (MOI) andthe cellular receptor used for infection. However, cholesteroldepletion of the viral envelope significantly reduced CDVinfectivity. Replenishment by addition of exogenous cholesterolrestored infectivity up to 80 percent. Thus, we conclude that CDVentry is dependent on cholesterol in the viral envelope.Furthermore, a reduced syncytium formation was observed when thecells were cholesterol depleted during the course of the infection.This may be related to the observation that the CDV envelopeproteins H and F partitioned into cellular detergent resistantmembranes (DRMs). Therefore, a role for lipid rafts during virusassembly and release as well is suggested. 176 pp. Englisch

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The Role of Lipid Rafts in Canine Distemper Virus Infection - Imhoff, Heidi
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Imhoff, Heidi:

The Role of Lipid Rafts in Canine Distemper Virus Infection - Taschenbuch

2008, ISBN: 9783639046304

[ED: Softcover], [PU: Vdm Verlag Dr. Müller], Cholesterol is known to play an important role in stabilising particular cellular membrane structures, so-called lipid rafts. For several viruses a dependence on cholesterol for virus entry and/or morphogenesis has been shown. Using flow cytometry and fluorescence microscopy we demonstrate that Canine distemper virus (CDV) infection of cells was not impaired after cellular cholesterol had been depleted by the drug methyl-beta-cyclodextrin (MbCD). This effect was independent of the multiplicity of infection (MOI) and the cellular receptor used for infection. However, cholesterol depletion of the viral envelope significantly reduced CDV infectivity. Replenishment by addition of exogenous cholesterol restored infectivity up to 80 percent. Thus, we conclude that CDV entry is dependent on cholesterol in the viral envelope. Furthermore, a reduced syncytium formation was observed when the cells were cholesterol depleted during the course of the infection. This may be related to the observation that the CDV envelope proteins H and F partitioned into cellular detergent resistant membranes (DRMs). Therefore, a role for lipid rafts during virus assembly and release as well is suggested.2008. 176 p. 220 mmVersandfertig in 3-5 Tagen, [SC: 0.00]

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The Role of Lipid Rafts in Canine Distemper Virus Infection - Imhoff, Heidi
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Imhoff, Heidi:
The Role of Lipid Rafts in Canine Distemper Virus Infection - Taschenbuch

2008

ISBN: 9783639046304

[ED: Softcover], [PU: Vdm Verlag Dr. Müller], Cholesterol is known to play an important role in stabilisingparticular cellular membrane structures, so-called lipid rafts. Forseveral viruses a dependence on cholesterol for virus entry and/ormorphogenesis has been shown. Using flow cytometry and fluorescencemicroscopy we demonstrate that Canine distemper virus (CDV)infection of cells was not impaired after cellular cholesterol hadbeen depleted by the drug methyl-beta-cyclodextrin (MbCD). Thiseffect was independent of the multiplicity of infection (MOI) andthe cellular receptor used for infection. However, cholesteroldepletion of the viral envelope significantly reduced CDVinfectivity. Replenishment by addition of exogenous cholesterolrestored infectivity up to 80 percent. Thus, we conclude that CDVentry is dependent on cholesterol in the viral envelope.Furthermore, a reduced syncytium formation was observed when thecells were cholesterol depleted during the course of the infection.This may be related to the observation that the CDV envelopeproteins H and F partitioned into cellular detergent resistantmembranes (DRMs). Therefore, a role for lipid rafts during virusassembly and release as well is suggested. 2008. 176 p. 220 mm Versandfertig in 3-5 Tagen, [SC: 0.00]

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The Role of Lipid Rafts in Canine Distemper Virus Infection - Heidi Imhoff
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ISBN: 9783639046304

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Cholesterol is known to play an important role in stabilisingparticular cellular membrane structures, so-called lipid rafts. Forseveral viruses a dependence on cholesterol for virus entry and/ormorphogenesis has been shown. Using flow cytometry and fluorescencemicroscopy we demonstrate that Canine distemper virus (CDV)infection of cells was not impaired after cellular cholesterol hadbeen depleted by the drug methyl-beta-cyclodextrin (MbCD). Thiseffect was independent of the multiplicity of infection (MOI) andthe cellular receptor used for infection. However, cholesteroldepletion of the viral envelope significantly reduced CDVinfectivity. Replenishment by addition of exogenous cholesterolrestored infectivity up to 80 percent. Thus, we conclude that CDVentry is dependent on cholesterol in the viral envelope.Furthermore, a reduced syncytium formation was observed when thecells were cholesterol depleted during the course of the infection.This may be related to the observation that the CDV envelopeproteins H and F partitioned into cellular detergent resistantmembranes (DRMs). Therefore, a role for lipid rafts during virusassembly and release as well is suggested. Buch / Broschur, [PU: VDM Verlag Dr. Müller, Saarbrücken]

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ISBN: 3639046307

ID: 4380959

VDM Verlag, [PU: VDM Verlag Dr. Müller, Saarbrücken]

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The Role of Lipid Rafts in Canine Distemper Virus Infection
Autor:

Imhoff, Heidi

Titel:

The Role of Lipid Rafts in Canine Distemper Virus Infection

ISBN-Nummer:

3639046307

Cholesterol is known to play an important role in stabilising particular cellular membrane structures, so-called lipid rafts. For several viruses a dependence on cholesterol for virus entry and/or morphogenesis has been shown. Using flow cytometry and fluorescence microscopy we demonstrate that Canine distemper virus (CDV) infection of cells was not impaired after cellular cholesterol had been depleted by the drug methyl-beta-cyclodextrin (MbCD). This effect was independent of the multiplicity of infection (MOI) and the cellular receptor used for infection. However, cholesterol depletion of the viral envelope significantly reduced CDV infectivity. Replenishment by addition of exogenous cholesterol restored infectivity up to 80 percent. Thus, we conclude that CDV entry is dependent on cholesterol in the viral envelope. Furthermore, a reduced syncytium formation was observed when the cells were cholesterol depleted during the course of the infection. This may be related to the observation that the CDV envelope proteins H and F partitioned into cellular detergent resistant membranes (DRMs). Therefore, a role for lipid rafts during virus assembly and release as well is suggested.

Detailangaben zum Buch - The Role of Lipid Rafts in Canine Distemper Virus Infection


EAN (ISBN-13): 9783639046304
ISBN (ISBN-10): 3639046307
Taschenbuch
Erscheinungsjahr: 2008
Herausgeber: VDM Verlag
176 Seiten
Gewicht: 0,278 kg
Sprache: eng/Englisch

Buch in der Datenbank seit 17.01.2008 10:57:09
Buch zuletzt gefunden am 13.09.2015 17:15:34
ISBN/EAN: 3639046307

ISBN - alternative Schreibweisen:
3-639-04630-7, 978-3-639-04630-4

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